Hsp90a, BioAssay(TM) Kit (Heat Shock Protein 90, Alpha)

Hsp90a is a molecular chaperone with essential functions in maintaining transformation. Inhibition of Hsp90a function has been shown to play a role in tumorigenesis and disease progression. The Hsp90a Assay Kit is designed for identification of Hsp90a inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin, an HSP90 inhibitor, for binding to purified recombinant Hsp90a.\n\nIntended Use:\nDesigned for identification of Hsp90a inhibitors using fluorescence polarization. The assay is based on the competition of fluorescently labeled geldanamycin, an HSP90 inhibitor, for binding to purified recombinant Hsp90a.\n\nTest Principle:\nThe Hsp90a inhibitor screening assay kit comes in a convenient 384-well format, with purified Hsp90a enzyme, FITC-labeled geldanamycin, and Hsp90a assay buffer for 400 enzyme reactions. The key to the Hsp90a Assay Kit is the fluorescently labeled geldanamycin. Using this kit, only one simple step on a microtiter plate is required for Hsp90a reactions. The FITC-labeled geldanamycin is incubated with a sample containing Hsp90a enzyme to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.\n\nKit Components:\nHsp90a recombinant enzyme: 1x70ug\nFITC-labeled geldanamycin (2.5uM): 1x30ul\nHsp90 assay buffer (5X): 1x4ml\nBlack, low binding NUNC microtiter plate: 1 plate\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137302

Size

500Tests

References

1. Kim J, Felts S, Lauger L, He H, Huezo H, Rosen N, and Chiosis G. J. Biomol. Screening 2004; 9(5): 375-381. 2. Howes R, Barril X, Dymock BW, Grant K, Northfield CJ, Robertson AGS, Surgenor A, Wayne J, Wright L, James K, Matthews T, Cheung KM, McDonald E, Workman P, Drysdale MJ. Anal. Biochem. 2006; 350:202-213.