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HSPA8 (Heat Shock Cognate 71kD Protein, Heat Shock 70kD Protein 8, HSPA10, HSP73, HSC70)

Cat no: H1831-28D

HSPA8 (Heat Shock Cognate 71kD Protein, Heat Shock 70kD Protein 8, HSPA10, HSP73, HSC70)

Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000\nWestern Blot: 1-3ug/ml, observed in lysates of cell line A431 on ~75kD bands\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H1831-28D

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Zhang Z, Fan J, Becker KG, Graff RD, Lee GM, Francomano CA. Comparison of gene expression profile between human chondrons and chondrocytes: a cDNA microarray study. Osteoarthritis Cartilage. 2006 May;14(5):449-59. Epub 2006 Jan 18.

Additional Info

Recognizes human HSPA8. Species sequence homology: Canine, mouse and rat.

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