HSPA9 (75kD glucose-regulated protein)

HSPA9(heat shock 70kDa protein 9 (mortalin)),also known as GRP75, mot-2, mthsp75, PBP74,\nHSPA9B, MORTALIN or MORTALIN, PERINUCLEAR, is a highly conserved member of the HSP70 \nfamily of proteins. It functions as a chaperone in the mitochondria, cytoplasm, and centrosome.\nThe HSPA9 gene is mapped to chromosome 5q31.2 based on an alignment of the HSPA9 sequence \nwith the genomic sequence. Knockdown of HSPA9 in erythroid cultures was associated with an \nincreased number of cells in the G0/G1 phase of the cell cycle and accelerated apoptosis. Knockdown \nof Hspa9 in mouse bone marrow cells, followed by transplantation into wildtype recipients, also resulted \nin loss of erythroid cell number. Haploinsufficiency for HSPA9 may contribute to abnormal \nhematopoiesis in myelodysplastic syndromes with deletions spanning chromosome 5q31.2. This protein \nplays a role in the control of cell proliferation. It may also act as a chaperone.\n\nUniProt Number:\nP38646\n\nGene ID:\nHSPA9\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144702

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Chen, T. H.-P., Kambal, A., Krysiak, K., Walshauser, M. A., Raju, G., Tibbitts, J. F., Walter, M. \nJ. Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in \nmice. Blood 117: 1530-1539, 2011.\n2. Gross, M. B. Personal Communication. Baltimore, Md. 9/12/2011.\n3. Kaul, S. C., Wadhwa, R., Matsuda, Y., Hensler, P. J., Pereira-Smith, O. M., Komatsu, Y., Mitsui, \nY. Mouse and human chromosomal assignments of mortalin, a novel member of the murine hsp70 \nfamily of proteins. FEBS Lett. 361: 269-272, 1995.

Additional Info

Recognizes human, mouse and rat HSPA9. No crossreactivity with other proteins.