HtrA1 (Serine Protease HTRA1, L56, Serine Protease 11, HTRA, PRSS11)

PRSS11; HTRA; IGFBP5-protease; L56; HtrA; ORF480; HtrA1, a 51kD protein, is a member of highly conserved mammalian HtrA serine protease family. It is a human homolog of bacterial HtrA and consists of a secretory signal sequence, an IGFBP-binding domain and a Kazal-type serine-protease inhibitor domain at the N-terminus and a highly conserved trypsin-like serine:protease domain and a PDZ domain at the C-terminus. It plays a major role in the regulation of cell growth. HtrA1 inhibits signaling mediated by TGF-beta family members and regulates many physiological processes. HtrA1 is up-regulated in placenta during pregnancy and plays an important role in implantation and placental physiology. It regulates the availability of IGFs by cleaving IGF-binding proteins. It acts as a tumor suppressor and is down-regulated in ovarian cancers and melanomas. Its role has been implicated in osteoarthritis suggesting a therapeutic intervention. Northern blot analysis detected high expression in placenta.\n\nApplications:\nSuitable for use in Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:250-1:1000\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H7952-30C

Size

100ug

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. De Luca A, De Falco M, Severino A, Campioni M, Santini D, Baldi F, Paggi MG, Baldi A. Distribution of the serine protease HtrA1 in normal human tissues. J Histochem Cytochem. 2003 Oct;51(10):1279-84. 2. Oka et.al Development 131, 1041-1053 (2004).

Additional Info

Recognizes human HtrA1. Species Crossreactivity: chimpanzee, bovine, canine, mouse, rat. Species sequence homology: chicken.