Human Brachiocephalic Artery Endothelial Cells are isolated from normal human brachiocephalic artery tissue. The cells are characterized by immunofluorescence with antibodies specific to Factor VIII-related antigen. T25 flasks is required for cell adhension to the culture vessels. Grow cells in ECM-coated culture vessels with 5% CO2. Each vial contains at least 1x10^6 cells per ml.