Human Primary Brachiocephalic Artery Smooth Muscle Cells are isolated from normal human brachiocephalic artery tissue. The cells are cryopreserved at passage 2 and delivered frozen. T25 flasks is required for cell adhension to the culture vessels. Grow cells in ECM-coated culture vessels with 5% CO2. Each vial contains at least 1x10^6 cells per ml.