Human Breast Tumor-Associated Endothelial Cells from Creative Bioarray are isolated from human breast tumor tissue. Human Breast Tumor-Associated Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray� Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.