HCFav from Creative Bioarray are isolated from the human heart. HCF-av are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCFav are characterized by immunofluorescent method with antibody to fibronectin. HCF-av are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-av are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.