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Human Dermal Epidermal Keratinocytes

Human Dermal Epidermal Keratinocytes

Cat no: CSC-C9225J

Supplier: Creative Bioarray
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Human Dermal Epidermal Keratinocytes from Creative Bioarray are isolated from normal human skin tissue. Human Dermal Epidermal Keratinocytes are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray�s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml. The method we use to isolate epidermal keratinocytes was developed based on a combination of established and our proprietary methods. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Catalogue number: CSC-C9225J
Size: 1.0 mL
Storage temp: We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 �C.

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