The Human Factor VIIa gla domainless was prepared from purified Human Factor VIIa usingCathepsin G. The Cathepsin G is removed using affinity chromatography after digestion.The above protein was purified from Human plasma that was tested and found negative byFDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, HBC Ab,HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors are screened for CJD(Creutzfeldt-Jakob Disease)