The Human Factor Xa beta gla domainless was prepared from HFX-GD using purified RVV-X.This RVV-X was removed after activation by gel filtration. Complete activation was observedon SDS PAGE. The above protein was purified from Human plasma that was tested andfound negative by FDA accepted methods for Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV,Syphilis, HBC Ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA. Donors arescreened for CJD (Creutzfeldt-Jakob Disease)