The CD Human IL-18 BPa ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-18 BPa in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human IL-18 BPa coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-18 BPa present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-18 BPa antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 BPa bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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