Human Keratocytes (HK) from Creative Bioarray are isolated from human cornea. HK are cryopreserved at primary culture. Each vial contains >5 x 10^5 cells in 1 ml volume. HK are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HK are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HK are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.