Human Mammary Microvascular Endothelial Cells from�Creative Bioarray�are isolated from human breast tissue. Human Mammary Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in�Creative Bioarray��Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded.�Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5�10^6�cells per ml.�The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of�magnetic beads�pre-coated with secondary antibody.