Human Metastatic Ovarian Carcinoma Epithelial Cells�are isolated from�human ovarian metastases tissue developed from colon cancer.�Human Metastatic Ovarian Carcinoma Epithelial Cells��are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in�Creative Bioarray�s�Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least �0.5x10^6�cells per ml. Cells can be expanded for 3-7 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.