The Human Multiplex Cytokine ELISA Kit is used for pro-inflammatory cytokines is designed for semi-quantitative and simultaneous determination of pro-inflammatory cytokines including interleukin 1? (IL-1 ?), interleukin 1? (IL-1?), interleukin 6 (IL-6), interleukin 8 (IL-8), granulocyte macrophage colony stimulating factor (GM-CSF), monocyte chemotactic and activating factor (MCAF), and tumor necrosis factor ? (TNF-?) in cell culture supernatant and other biological samples.