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Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit

Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit

Cat no: KTE61211

Supplier: Abbkine Scientific Co.Ltd.
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Glycosylphosphatidylinositol (GPI) is used as a membrane anchor by many eukaryotic cell surface proteins. The first step in GPI biosynthesis involves the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI) . The second step is de-N-acetylation of GlcNAc-PI. PIGL encodes a deduced 252-amino acid protein that shares 77% identity with the rat protein. Using transfection into mammalian PIGL-deficient cells, Watanabe et al. (1999) demonstrated that S. cerevisiae Gpi12 is the ortholog of human and rat PIGL. Disruption of Gpi12 in yeast resulted in a lethal phenotype. Using purified, recombinant rat PIGL, Watanabe et al. (1999) demonstrated that PIGL has GlcNAc-PI de-N-acetylase activity in vitro, which is enhanced by metal ions.
Catalogue number: KTE61211
Reactivities: Human
Applications: ELISA
Size: 48T, 96T, 96T*5, 96T*50
Accession: Q9Y2B2
Gene: 9487
Additional info: Human N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIGL. No significant cross-reactivity or interference between Human PIGL and analogues was observed.

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