HNPC from Creative Bioarray are isolated from nucleus pulposus of human intervertibral disc. HNPC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HNPC are characterized by immunofluorescent method with antibodies to fibronectin and vimentin. HNPC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HNPC are guaranteed to further expand for 15 population doublings in the condition provided by Creative Bioarray.