Human Peridontal Ligament Fibroblasts(HPLF) from Creative Bioarray are isolated from human periodontal tissue. HPDLF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPDLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HPDLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPDLF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.