Human CD4+CD45RO+ memory T cells from Creative Bioarray are isolated from peripheral blood mononuclear cells by depleting cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD45RA, CD56, CD123, TCR?/?, and CD235a are from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+CD45RO+ memory T cells.