High Sensitivity Prostaglandin E2 (PGE2) Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva and tissue culture media samples. A PGE2 standard is provided to generate a standard curve for the assay. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse IgG. A PGE2-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to PGE2. After an overnight incubation at 4�C, the plate is washed and substrate is added. The substrate reacts with the bound PGE2-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the PGE2 in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
Prostaglandins are synthesized from arachidonic acid by cyclooxygenase (COX)-1 or -2, which convert the acid into PGH2. This is further processed by cytosolic or microsomal prostaglandin synthases to become PGE2 or one of several other prostanoids. Prostacyclin is the major cyclooxygenase product in blood vessel walls and it is present in inflammatory fluids in similar concentrations to PGE2. PGE2 is a potent vasodilator and produces hyperalgesia. PGE2 is produced by a wide variety of tissues and in several pathological conditions, including inflammation, arthritis, fever, tissue injury, endometriosis, and a variety of cancers.
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