The Human Prealbumin ELISA kit is designed for detection of human Prealbumin in plasma, serum, urine, saliva, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures prealbumin in less than 4hours. A polyclonal antibody specific for prealbumin has been pre-coated onto a microplate. Prealbumin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for prealbumin, which is recognized by a streptavidinperoxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured
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