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Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit

Cat no: KTE61326

Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit

P4HA3 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. P4HA3 has all residues necessary to bind the peptide substrate, to coordinate Fe(2+) and the 2-oxoglutarate cosubstrate, and for enzymatic activity. Processed P4HA3 contains 525 amino acids and shares 91% and 94% amino acid identity with rat and mouse P4ha3, respectively, and 35% and 37% identity with P4HA1 and P4HA2, respectively. Northern blot analysis detected a 2.7-kb P4HA3 transcript. Highest expression was in placenta, adult liver, and fetal skin, with lower levels in fetal liver, lung, and muscle. RT-PCR detected P4HA3 expression in all tissues examined, with highest expression in placenta and fetal kidney, liver, and lung. Expression of P4HA3 was much lower than that of P4HA1.

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SPECIFICATIONS

Catalog Number

KTE61326

Size

48T, 96T, 96T*5, 96T*50

Applications

ELISA

Reactivities

Hum

Gene Id

283208

Accession

Q7Z4N8

Additional Info

Human Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) ELISA Kit has high sensitivity and excellent specificity for detection of Human P4HA3. No significant cross-reactivity or interference between Human P4HA3 and analogues was observed.

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SUPPLIER INFO

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