The Human Protein C ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Protein C in urine, saliva, milk, and cell culture supernatant. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Protein C in less than 4 hours. A polyclonal antibody specific for human Protein C has been pre-coated onto a 96-well microplate with removable strips. Protein C in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for Protein C, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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