The Human Protein C ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Protein C in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human Protein C in less than 3 hours. A polyclonal antibody specific for human Protein C has been pre-coated onto a 96-well microplate with removable strips. Protein C in standards and samples is competed with a biotinylated Protein C sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.