Human Skeletal Muscle Cells (HSkMC) are isolated from the limbal skeletal muscle. They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC have been used in the study of genetic muscular diseases such as Malignant Hyperthermia. HSkMC also have the potential to act as a cardiac graft, mending damage to the heart. Recently, cultured Human Skeletal Muscle cells have been used in micro gravity experiments to study the effects of low gravity environments on Human Skeletal Muscle.