Th17 cells are a subset of CD4+ T cells defined by their ability to produce the cytokines IL-17, IL-17F and IL-22. Th17 cells participate in normal epithelial immunity, their dysregulation is implicated in psoriasis, multiple sclerosis, systemic lupus erythmatosis, rheumatoid arthritis, and inflammatory bowel disease. BioLegend’s human Th17 flow kit is designed and formulated specifically for one-step intracellular immunofluorescence staining and flow cytometric analysis of Th17 cells in a mixed cell population. This kit is composed of fluorochrome conjugated anti-human CD3, CD4 and IL-17 antibodies, isotype control and the critical buffers. It is easy to use for identification of Th17 cells.
Materials provided: 1. Mouse IgG1,k isotype control Alexa Fluor(R) 647/CD3 FITC/CD4 PE,25 tests2. Anti-human IL-17 Alexa Fuor(R) 647/CD3 FITC/CD4 PE, 25 tests 3. Fixation Buffer, 100ml, Cat. No. 4208014. Permeabilization Wash Buffer (10X), 100 ml, Cat. No. 421002 Materials not included:Cell Staining Buffer (Cat. No. 420201)