Humanin (HN)

Humanin is a novel 24aa polypeptide identified in Alzheimer's disease-affected brain. It is 99% homologous to mitochondrial 16s ribosomal RNA and functions as a novel neuroprotective and cytoprotective factor. It specifically inhibits neuronal cell death induced by beta-amyloids and Alzheimer's disease causing FAD genes by competitively inhibiting access of FPRL1 receptor to beta-amyloids. It acts as an anti-apoptotic factor by binding to Bcl-2/Bax family of pro-apoptotic factors including Bax, Bak, Bid and BimEL and inhibits their pro-apoptotic activity. It activates JAK/STAT signaling pathway and is regulated by TRIM11. It interacts with IGFBP-3 and specifically blocks IGFBP-3 induced cell death in glioblastoma cells. Humanin is also expressed in colon and testis.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100-1:1000\nWestern Blot: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRecombinant fusion protein\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H7953-30F1

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Bin Guo, Dayong Zhai, Edelmira Cabezas, Kate Welsh, Shahrzad Nouraini, Arnold C. Satterthwait & John C. Reed. Humanin peptide suppresses apoptosis by interfering with Bax activation NATURE, VOL 423: 22 MAY 2003 2. Hashimoto et.al PNAS 98(11):6336-41 (2001 May 22) 3. Tajima et.al Neurosci Lett., 324(3):227-31 (2002 May 24) 4. Maaria et.al PNAS 100 (22); 13042-13047 (October 28, 2003) 5. Ying et.al The Journal of Immunology, 172: 7078-7085 (2004) 6. Zhai et.al J. Biol. Chem., 280 (16); 15815-15824 (April 22, 2005) 7. Luciano et.al J. Biol. Chem., 280 (16);15825-15835 (April 22, 2005).

Additional Info

Recognizes human Humanin.