Huntingtin Protein, aa2703-2911 (Huntington Disease)

Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.\n\nApplications:\nSuitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Frozen. Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control Tissue:\nBrain\n\nRecommended Secondary Antibody:\nI1904-65W: IgG, F(ab

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SPECIFICATIONS

Catalog Number

H7960-01

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rabbit

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Wilkinson, F.L., et al., Molecular Brain Research. 69: 10-20 (1999).

Additional Info

Recognizes an epitope corresponding to the human HDC region (2703-2911 amino acids) of the Huntingtin protein. Detects a 350kD band on Western Blots but also detects smaller degradation products of Huntingtin. Recognizes both denatured and native Huntingtin in human brain. The combined use of H7960-01, H7960 and H7960-01A demonstrate that huntingtin is enriched in neuronal cells in the brain. Species Crossreactivity: rabbit and mouse