HuR (ELAVL1)

Hu-antigen R (HuR), also know as ELAVL1, is a ubiquitously expressed member of the embryonic lethal abnormal vision (ELAV) family of RNA-binding proteins. Three other closely related neural proteins, called HelN1 (or HuB), HuC and HuD, are target antigens in paraneoplastic neuropathy. HuR is relatively low abundance, about 5x10e5 molecules per HeLa cell. Overexpression of HuR in transiently transfected mammalian cells leads to stabilization of reporter mRNAs carrying AU-rich elements (AREs) in their 3' untranslated regions. Although immunofluorescence studies show HuR to be predominantly nuclear in interphase cells, it has been demonstrated to shuttle between the nucleus and cytoplasm. Its shuttling signal, HNS, located in the hinge region between its second and third RRMs, appears similar but not identical to the M9 shuttling sequence first identified in hnRNP A1. It has been suggested that HuR may initially bind ARE-containing mRNAs in the nucleus and accompany them to the cytoplasm, providing ongoing protection from the decay machinery. A recent report indicates that overexpression of HuR in ovarian carcinoma is associated with increased COX-2 expression, and is a prognostic factor.\n\nApplications: \nSuitable for use in Western Blot, ELISA, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:20-1:100\nELISA: 1:100-1:1000\nImmunoprecipitation (Native): 1:10-1:20\nImmunofluorescence: 1:20-1:100\nImmunohistochemistry (Paraffin): 1:100-1:1000. Requires heat induced epitope retrieval (HIER) using citrate buffer in a pressure cooker for 5 minutes (1).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H7953-34A

Size

1ml

Applications

ELISA, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.1% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1,k

References

1. Denkert, C., et al., Cancer Res. 64(1): 189-195 (2004). 2. Gallouzi, I.E., et al., PNAS 97(7): 3073-3078 (2000). 3. Ma, W., et al., J. Biol. Chem. 271: 8144-8151 (1996).

Additional Info

Recognizes human HuR, HuB, HuC and HuD proteins at ~34-36kD. Reactivity has been confirmed with human OVCAR-3, SKOV-3, CAOV-3, ES-2, PA-1, OAW42, Mdah2774, PA1, A27/80, EFO-27, and HeLa cells and human testis, spleen, skeletal muscle, lung, liver, kidney, heart, and brain homogenates by Western Blot, HeLa cells by Immunoprecipitation and Immunofluorescence and Ovarian carcinoma tissues by Immunohistochemistry.