Hyaluronic Acid Binding Protein 1, Recombinant, Mouse (Hyaluronan Binding Protein 1, HABP1, C1qBP, C1q-R, Human complement component 1, q subcomponent binding protein)

Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208 aa mature region (3). The 3 kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively. HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6-9). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10-16). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (17).\n\nA DNA sequence encoding the mature mouse HABP1 (Leu 72-Gln 279; Accession # NP_031599), fused with 2X Met at the N-terminus and a six-histidine tag at the C-terminus, was expressed in E. coli.\n\nCalculated molecular mass of ~ 24.8kD.\n\nActivity: \nMeasured by its ability to bind human C1q.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H7980-36

Size

50ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute with PBS to (same/more than)100ug/ml.

Purity

(same/more than) 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: (same/less than)1EU/1ug.

References

1. Thakur, S.C. et al., 2006, J. Androl. 27:604. \n2. Peerschke, E.I.B. and B. Ghebrehiwet, 2007, Immunobiology 212:333. 12. Sunayama, J. et al., 1994, Cell Death Differ. 11:771.\n3. Lynch, N.J. et al., 1997, FEBS Lett. 418:111. 13. Pupo, A.S. and K.P. Minneman, 2003, J. Recept. Signal Transduct.\n4. Jiang, J. et al., 1999, Proc. Natl. Acad. Sci. 96:3572. Res. 23:185.\n5. Rozanov, D.V. et al., 2002, J. Biol. Chem. 277:9318. 14. Schaerer, M.T. et al., 2001, J. Biol. Chem. 276:26597.\n6. Deb, T.B. and K. Datta, 1996, J. Biol. Chem. 271:2206. 15. Peterson-Mahrt, S.K. et al., 1999, EMBO J. 18:1014.\n7. Lim, B.L. et al., 1996, J. Biol. Chem. 271:26739. 16. Chattopadhyay, C. et al., 2004, Nucleic Acids Res. 32:3632.\n8. Grebrehiwet, B. et al., 1994, J. Exp. Med. 179:1809. 17. Majumdar, M. et al., 2002, Biochem. Biophys. Res. Commun.\n9. Waggoner, S.N. et al., 2007, J. Leukoc. Biol. 82 epub. 291:829.\n10. Innamorati, G. et al., 2006, Cell. Signal. 18:761.\n11. Robles-Flores, M. et al., 2002, J. Biol. Chem. 277:5247.