Hyaluronic Acid Binding Protein, Bovine (Hyaluronan Binding Protein, HABP)

Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208 aa mature region (3). The 3kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively. HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6-9). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10-16). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (17).\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCertificate of Origin:\nThe raw animal product (Bovine nasal cartilage) used in the manufacturing of Hyaluronic Acid Binding Protein is derived from materials originating in North America and collected in USDA approved facilities and inspected to be free of disease.\n\nAlso Available:\nH7980-30A: Hyaluronic Acid Binding Protein (HABP) Pab Rb x Bo\nH7980-35: Hyaluronic Acid Binding Protein (HABP) (Biotin)

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SPECIFICATIONS

Catalog Number

H7980-30

Size

100ug

Applications

ELISA

Form

Supplied as a white lyophilized powder from 0.125% sodium phosphate, 0.85% sodium chloride, 1% BSA.\nReconstitute in 100ul sterile PBS.

Purity

(same/more than) 90% (SDS-PAGE); 3 bands at 70-80kD, 40-45kD, 45kD

References

US Biological application references: 1. Campo, G.M. et al., (2012) Innate Immunity, January 25, 2012, doi: 10.1177/1753425911435953. 2. Campo, G. M. et al., (2012) Gene, 494; 24-35. 1. Guan, H., et al., J. Immunology 179: 3715-3723 (2007). 2. Thakur, S.C., et al., J. Androl. 27: 604 (2006). 3. Peerschke, E.I.B. and Ghebrehiwet, B., Immunobiology 212: 333 (2007). 4. Lynch, N.J., et al., FEBS Lett. 418: 111 (1997). 5. Jiang, J., et al., Proc. Natl. Acad. Sci. 96: 3572 (1999). 6. Rozanov, D.V., et al., J. Biol. Chem. 277: 9318 (2002). 7. Deb, T.B. and Datta, K., J. Biol. Chem. 271: 2206 (1996). 8. Lim, B.L., et al., J. Biol. Chem. 271: 26,739 (1996). 9. Grebrehiwet, B., et al., J. Exp. Med. 179: 1809 (1994). 10. Waggoner, S.N., et al., J. Leukoc. Biol. 291: 82 epub. 11. Innamorati, G., et al., Cell. Signal. 18: 761 (2006). 12. Robles-Flores, M., et al., J. Biol. Chem. 277: 5247 (2002). 13. Laemmli, U.K., Nature (Lond.) 227: 680-685 (1970). 14. Rao, C.M., et al., Journal of Parasitology 85(4): 682-687 (1999). 15. Sunayama, J., et al., Cell Death Differ. 11: 771 (1994). 16. Pupo, A.S. and Minneman, K.P., J. Recept. Signal Transduct. Res. 23: 185 (2003). 17. Schaerer, M.T., et al., J. Biol. Chem. 276: 26,597 (2001). 18. Peterson-Mahrt, S.K., et al., 1999, EMBO J. 18: 1014 (1999). 19. Chattopadhyay, C., et al., Nucleic Acids Res. 32: 3632 (2004). 20. Majumdar, M., et al., Biochem. Biophys. Res. Commun. 291: 829 (2002).