Hyaluronic Acid Binding Protein (Hyaluronan Binding Protein, HABP) (Biotin)

Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208 aa mature region (3). The 3kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively. HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6-9). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10-16). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (17).\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nSDS-PAGE: Recognizes bands at 70, 42 and 33kD.\nELISA: Binds streptavidin\nOptimal dilutions to be determined by the researcher.\n\nHA (Hyaluronic acid) binding activity [Sandwich ELISA]:\nA450/630nm: 1.0 (HA-800ng/ml)\nA450/630nm: 0.25 (HA-0ng/ml)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCertificate of Origin:\nThe raw animal product (Bovine nasal cartilage) used in the manufacturing of Hyaluronic Acid Binding Protein is derived from materials originating in North America and collected in USDA approved facilities and inspected to be free of disease.\n\nAlso Available:\nH7980-30: Hyaluronic Acid Binding Protein (HABP)\nH7980-30A: Hyaluronic Acid Binding Protein (HABP) Pab Rb xBo\nH7980-35: Hyaluronic Acid Binding Protein (HABP) (Biotin)

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SPECIFICATIONS

Catalog Number

H7980-35

Size

50ug

Applications

ELISA, IHC

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA. Labeled with Biotin. Reconstitute in 100ul sterile ddH2O.

Purity

(same/more than) 90% (SDS-PAGE); 3 bands at ~70, 42kD, 33kD

References

USBio application reference: 1. Guan, H., et al., J. Immunology 179: 3715-3723 (2007) (IHC - mice). 2. Carrette, O., et al., Biology of Reproduction 65: 301-308 (2001). 3. Lopez, J.I., et al., (2005) Cancer Res 65: 6755. 4. Cargill, R. et al., (2011) Neurobiology of Aging, doi:10.1016/j.neurobiolaging.2011.07.006. \nGeneral References:\n1. Thakur, S.C. et al., 2006, J. Androl. 27:604. 2. Peerschke, E.I.B. and B. Ghebrehiwet, 2007, Immunobiology 212:333. 12. Sunayama, J. et al., 1994, Cell Death Differ. 11:771. 3. Lynch, N.J. et al., 1997, FEBS Lett. 418:111. 13. Pupo, A.S. and K.P. Minneman, 2003, J. Recept. Signal Transduct. 4. Jiang, J. et al., 1999, Proc. Natl. Acad. Sci. 96:3572. Res. 23:185. 5. Rozanov, D.V. et al., 2002, J. Biol. Chem. 277:9318. 14. Schaerer, M.T. et al., 2001, J. Biol. Chem. 276:26597. 6. Deb, T.B. and K. Datta, 1996, J. Biol. Chem. 271:2206. 15. Peterson-Mahrt, S.K. et al., 1999, EMBO J. 18:1014. 7. Lim, B.L. et al., 1996, J. Biol. Chem. 271:26739. 16. Chattopadhyay, C. et al., 2004, Nucleic Acids Res. 32:3632. 8. Grebrehiwet, B. et al., 1994, J. Exp. Med. 179:1809. 17. Majumdar, M. et al., 2002, Biochem. Biophys. Res. Commun. 9. Waggoner, S.N. et al., 2007, J. Leukoc. Biol. 82 epub. 291:829. 10. Innamorati, G. et al., 2006, Cell. Signal. 18:761. 11. Robles-Flores, M. et al., 2002, J. Biol. Chem. 277:5247. 12. Laemmli, U.K., Nature (Lond.) 227: 680-685 (1970). 13. Rao, C.M., Salotra, P., Datta, K.: Possible Role of the 34-Kilodalton Hyaluronic Acid-Binding Protein in Visceral Leishmaniasis. Journal of Parasitology 85: 682-687 (1999).