Hydroxycarboxylic Acid Receptor 3 (HCAR3) ELISA Kit

Cat no: ABIN422327

Supplier: Antibodies-online

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Hydroxycarboxylic Acid Receptor 3 (HCAR3) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Coupled Receptor 109B (GPR109B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Coupled Receptor 109B (GPR109B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Coupled Receptor 109B (GPR109B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Catalogue number:	ABIN422327
Size:	96 tests
Accession:	P49019
Gene:	8843
Antigen:	GPR109B
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range:	0.156-10 ng/mL
Sample type:	Tissue Homogenate,Cell Lysate,Biological Fluids
Entrez gene:	8843

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Hydroxycarboxylic Acid Receptor 3 (HCAR3) ELISA Kit

Cat no: ABIN422327

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