Hydroxyguanosine 8 (8-OHG)

Reactive oxygen species (ROS) are formed at high levels as by-products of the normal cellular metabolism. Both nuclear and mitochondrial DNA has been shown to accumulate high levels of 8-hydroxy-2'-deoxyguanosine, a very stable and damaging product of hydroxylation of guanine at carbon 8. 8-hydroxyguanosine (8-OHG) induces transversion of G to T, which is potentially mutagenic. The base excision repair (BER) pathway is the most important cellular protection mechanism responding to oxidative DNA damage. They remove modified DNA bases before they are incorporated into DNA during replication. The key enzymes MutT homologs (MutT/MTH) in the BER process are DNA glycosylases, which remove different damaged bases by cleavage of the N-glycosylic bonds between the bases and the deoxyribose moieties of the nucleotide residues. The 8-oxoG glycosylases (Fpg or MutM/OGG) and the MutY homologs (MutY/MYH) glycosylases along with MutT/MTH protect cells from the mutagenic effects of 8-oxoG. 8-hydroxyguanosine (8-OHG) has been used as oxidative stress marker.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: Rat heart\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H9076

Size

100ul

Applications

ELISA, IF, IHC

Hosts

Goat

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Ghosh S 2006 Free Radical Biology and Medicine, 41, 1413-1424, IF rat heart. 2. PNAS 89: 3375-3379 (1992). 3. J. Neurosci. 19, 1959-1964. 4. Gene 286: 127-34 (2002). 5. Cell. Biochem. Biophys. 35: 141 (2001). 6. Mutat. Res. 513(1-2): 37-48 (2002). 7. Free Radic. Biol. Med. 28(1): 13 (2000). 8. Free Radic. Biol. Med. 11-12: 1251-8 (1999).

Additional Info

Recognizes 8-hydroxy-2'-dexoyguanosine (oh8dG), 8-hydroxyguanine (oh8G) and 8-hydroxyguanosine from all species.