Hydroxyguanosine 8 (8-OHG)

Reactive oxygen species (ROS) are formed at high levels as by-products of the normal cellular metabolism. Both nuclear and mitochondrial DNA has been shown to accumulate high levels of 8-hydroxy-2'-deoxyguanosine, a very stable and damaging product of hydroxylation of guanine at carbon 8. 8-hydroxyguanosine (8-OHG) induces transversion of G to T, which is potentially mutagenic. The base excision repair (BER) pathway is the most important cellular protection mechanism responding to oxidative DNA damage. They remove modified DNA bases before they are incorporated into DNA during replication. The key enzymes MutT homologs (MutT/MTH) in the BER process are DNA glycosylases, which remove different damaged bases by cleavage of the N-glycosylic bonds between the bases and the deoxyribose moieties of the nucleotide residues. The 8-oxoG glycosylases (Fpg or MutM/OGG) and the MutY homologs (MutY/MYH) glycosylases along with MutT/MTH protect cells from the mutagenic effects of 8-oxoG. 8-hydroxyguanosine (8-OHG) has been used as oxidative stress marker.\n\nApplications: \nSuitable for use in ELISA Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.1-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H9076-01

Size

50ug

Applications

ELISA

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein A/G chromatography

Isotype

IgG2a

References

1. Li J 2007 J. Clin. Invest., Nov 2007; 117: 3283-3295 IHC, mouse brain 2. Manczak M 2005 J. Neurochemistry 92,494-504, IHC, mouse brain 3. PNAS 89: 3375-3379 (1992). 4. J. Neurosci. 19, 1959-1964. 5. Gene 286: 127-34 (2002). 6. Cell. Biochem. Biophys. 35: 141 (2001). 7. Mutat. Res. 513(1-2): 37-48 (2002). 8. Free Radic. Biol. Med. 28(1): 13 (2000). 9. Free Radic. Biol. Med. 11-12: 1251-8 (1999).

Additional Info

Recognizes 8-hydroxy-2'-dexoyguanosine (oh8dG), 8-hydroxyguanine (oh8G) and 8-hydroxyguanosine from all species.