Hydroxyproline BioAssay(TM) Kit

Hydroxyproline (4-hydroxyproline) is a common nonproteinogenic amino acid. It is found only in collagen and elastin in mammals but exists in a number of other proteins in plants. Hydroxyproline is formed only as a post-translational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline. Hydroxyproline in tissue hydrolysates is a direct measure of the amount of collagen or gelatin present. A variety of disease states are believed to affect collagen turnover and can cause elevated serum or urine hydroxyproline. Such conditions range from neoplastic, inflammatory, renal or bone disease to endocrine and autoimmune disorders. This Hydroxyproline Assay Kit is designed to measure hydroxyproline in tissue or protein/peptide hydrolysates. It can be used to measure hydroxyproline from other biological samples such as serum or urine if they have undergone a prior purification process. It is an easy, convenient method which results in a chromogen with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-2ug.\n\nKit components: \nOxidation Buffer, 1x10ml\nChloramine T Concentrate, 1x600ul\nPerchloric acid/Isopropanol Solution, 1x5ml\nDMAB Concentrate (in DMSO), 1x5ml\nHydroxyproline Standard (1mg/ml), 1x100ul\n\nStorage and Stability:\nStore kit at 4 degrees C, protect from light. The reagent concentrates are stable as supplied. Stable for at least 6 months. Once the concentrates have been diluted to working concentration, they are only good for 2-3 hours so only make as enough each reagent as necessary for the number of samples and standards to be quantified. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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