Hydroxysteroid Dehydrogenase, 11-beta, Type II (11b-HSD2)

11 beta-HSD2 plays a critical role in normal physiology in the corticosteroid regulation of sodium homeostasis and the pathophysiology of hypertension by converting active cortisol to inactive cortisone. Mutation in the gene encoding 11 beta-HSD2 results in cortisol induction of hypertension and hypokalemia, a syndrome of apparent mineralocorticoid excess. 11 beta-HSD2 mRNA and protein are expressed in placenta and mineralocorticoid target tissues, particularly the kidney. In mouse, expression of this enzyme is tissue-specific and developmentally regulated, indicating the importance of the enzyme in controlling glucocorticoid- mediated maturation. \n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 1:200 (5ug/ml)\nOptimal dilutions to be determined by the researcher. \n\nPositive Control:\nKidney, Caco-2 and HT-29 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H9117-50R

Size

150ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in TBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1 Quinkler, M., Stewart, P.M Journal of Clinical Endocrinology & Metabolism 88(6) 2384-2392 (2003). 2 White, P.C., Mune, T., Agarwal, A.K. Endocr Rev 18(1) 135-156 (1997). 3 Thompson, A., Han, V.K.M., Yang, K. J Steroid Biochem Molec Biol 88 367-375 (2004).

Additional Info

Recognizes human 11 beta-HSD2 at ~44kD. Species Crossreactivity: mouse and rat.