Hydroxysteroid Dehydrogenase, 11-beta Type II (HSD)

11-b-hydroxysteroid dehydrogenase (11b-HSD) is a microsomal short chain dehydrogenase/reductase which catalyzes the inter-conversion of biologically active glucocorticoid (cortisol in human, corticosterone in rats and mice) and inactive glucocorticoid (cortisone and 11-dehydrocorticosterone). Two tissue specific isoforms (11b-HSD1 and 11b-HSD2) of 11b-HSD with two different functions regarding glucocorticoid availability, have been identified. The decreased 11b-hydroxy oxidation of cortisol results in Apparent Mineralocorticoid Excess (AME) disorder. AME is principally a disorder of juveniles and children with this condition oxidize cortisol to cortisone poorly but carry out the reverse process unimpaired. AME arises from mutations in the 11b-HSD2 gene. The glucocorticoids can also be produced locally by 11b-HSD1 and increased visceral accumulation of glucocorticoids results in visceral obesity, insulin resistant diabetes, hyperlipidemia and hyperphagia. 11betaHSD-2 (rat 400aa, mouse 396aa, human 405aa) is a ~41kD glycosylated membrane-protein present in the endoplasmic reticulum (ER). The N-terminal and C-terminal (catalytic domain) of 11b-HSD2 are in the lumen and cytoplasm of ER, respectively. 11b-HSD2 irreversibly catalyzes the dehydrogenation of active 11b-hydroxycorticoids before they occupy mineralocorticoid receptors (MR) and thus confers aldosterone selectivity for inherently nonselective MR. The enzyme is expressed in a wide array of tissues, with highest level mineralocorticoid target cells such as the renal and outer medullary collecting ducts. In mouse, rat and human, the over-all aa seq of 11b-HSD2 is >80% identical. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml using ECL technique.\nELISA: 1:10,000-1:100,000 using 50-100ng of H9117-82 control peptide per well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H9117-80

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Zhou MY, et al. 1995. Endocrinol. 136:3729-3734. 2. Albiston AL, et al. 1994. Mol. Cell. Endocrinol. 105:R11. 3. Agarwal AK, et al. 1995. Genomics 29:195. 4. Brown RW, et al. 1996. Biochem. J. 313:1007. 5. Mune T, et al. 1995. Nat. Genet. 10:394. 6. Masuzaki, et al. 2001. Science 294:2166. 7. Odermatt, et al. 1999. J. Bio. Chem. 274:28762. 8. Blum, et al. 2000. BBRC. 276:428.

Additional Info

Recognizes mouse Hydroxysteroid Dehydrogenase, 11-beta Type II (HSD).