Hydroxysteroid Dehydrogenase, 11-beta Type II (HSD)

11-b-hydroxysteroid dehydrogenase (11b-HSD) is a microsomal short chain dehydrogenase/reductase (SDR) which catalyzes the inter-conversion of biologically active glucocorticoid (cortisol in human and corticosterone in rats and mice) and inactive glucocorticoid (cortisone and 11-dehydrocorticosterone). Two tissue specific isoforms (11beta-HSD1 and 11beta-HSD2) of 11b-HSD with two different functions regarding glucocorticoid availability, have been identified. The decreased 11b-hydroxy oxidation of cortisol results in Apparent Mineralocorticoid Excess (AME) disorder which is manifested by hypertension, hypokalemia, low plasma renin activity, and responsiveness to spironolactone. AME is principally a disorder of juveniles and children with this condition oxidize cortisol to cortisone poorly but carry out the reverse process unimpaired. AME arises from mutations in the 11beta-HSD2 gene. The glucocorticoids can also be produced locally by 11b-HSD1 and increased visceral accumulation of glucocorticoids results in visceral obesity, insulin resistant diabetes, hyperlipidemia and hyperphagia.\n11betaHSD-2 (rat 400-aa, mouse 396-aa, human 405-aa) is a ~41kD glycosylated membrane-protein present in the endoplasmic reticulum (ER). The N-terminal and C-terminal (catalytic domain) of 11b-HSD2 are in the lumen and cytoplasm of ER, respectively. 11b-HSD2 irreversibly catalyzes the dehydrogenation of active 11b-hydroxycorticoids before they occupy mineralocorticoid receptors (MR) and thus confers aldosterone selectivity for inherently nonselective MR. The enzyme is expressed in a wide array of tissues, with highest level mineralocorticoid target cells such as the renal and outer medullary collecting ducts. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 \nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nH9117-82: Hydroxysteroid Dehydrogenase, 11-beta Type II, Control Peptide, Mouse (HSD)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H9117-81

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

(1) Zhou MY et al (1995) Endocrinol. 136, 3729-3734; Albiston AL et al (1994) Mol. Cell. Endocrinol. 105, R11; Agarwal AK et al (1995) Genomics 29, 195, Brown RW et al (1996) Biochem. J. 313, 1007, Mune T et al (1995) nat. Genet. 10, 394; Masuzaki et al. (2001) Science 294, 2166; Odermatt et al. (1999) J. Bio. Chem. 274, 28762; Blum et al. (2000) BBRC. 276, 428.

Additional Info

Recognizes mouse 11b-HSD2. No significant sequence homology with 11b-HSD1 or other proteins.