Hydroxysteroid Dehydrogenase, 11-beta Type II, Human (11b-HSD2) (Control Peptide)

Control Peptide for H9117-50G (antiserum) and H9117-50H (affinity purified) antibodies.\n11-b-hydroxysteroid dehydrogenase (11b-HSD) is a microsomal short chain dehydrogenase/reductase (SDR) which catalyzes the inter-conversion of biologically active glucocorticoid (cortisol in human and corticosterone in rats and mice) and inactive glucocorticoid (cortisone and 11-dehydrocorticosterone). Two tissue specific isoforms (11b-HSD1 and 11b-HSD2) of 11b-HSD with two different functions regarding glucocorticoid availability, have been identified. The decreased 11b-hydroxy oxidation of cortisol results in Apparent Mineralocorticoid Excess (AME) disorder which is manifested by hypertension, hypokalemia, low plasma renin activity, and responsiveness to spironolactone. AME is principally a disorder of juveniles and children with this condition oxidize cortisol to cortisone poorly but carry out the reverse process unimpaired. AME arises from mutations in the 11b-HSD2 gene. The glucocorticoids can also be produced locally by 11b-HSD1 and increased visceral accumulation of glucocorticoids results in visceral obesity, insulin resistant diabetes, hyperlipidemia and hyperphagia. 11bHSD-2 (rat 400-aa, mouse 396-aa, human 405-aa) is a ~41kD glycosylated membrane-protein present in the endoplasmic reticulum (ER). The N-terminal and C-terminal (catalytic domain) of 11b-HSD2 are in the lumen and cytoplasm of ER, respectively. 11b-HSD2 irreversibly catalyzes the dehydrogenation of active 11b-hydroxycorticoids before they occupy mineralocorticoid receptors (MR) and thus confers aldosterone selectivity for inherently nonselective MR. \n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng control peptide/well.\nAntibody Blocking: 5-10ug per 1ug H9117-50H (affinity purified) or per 1ul H9117-50G (antiserum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H9117-50L

Size

50ug

Form

Supplied as a liquid in PBS, pH 7.5.

Purity

Highly purified

References

1. Zhou MY et al (1995) Endocrinol. 136, 3729-3734. 2. Albiston AL et al (1994) Mol. Cell. Endocrinol. 105, R11. 3. Agarwal AK et al (1995) Genomics 29, 195. 4. Brown RW et al (1996) Biochem. J. 313, 1007. 5. Mune T et al (1995) nat. Genet. 10, 394. 6. Masuzaki et al. (2001) Science 294, 2166;. 7. Odermatt et al. (1999) J. Bio. Chem. 274, 28762. 8. Blum et al. (2000) BBRC. 276, 428.