Hydroxysteroid Dehydrogenase, 17-beta Type I, CT, (HSD, 17-beta Hydroxysteroid Dehydrogenase Type 1, 17-beta HSD1, HSD17B1, EDH17B2, EDHB17, Estradiol 17-beta Dehydrogenase 1, HSD17, Hydroxysteroid (17-beta) Dehydrogenase 1, Placental 17-beta Hydroxystero

The principal human estrogen, 17 beta-estradiol, is a potent stimulator of certain endocrine-dependent forms of breast cancer. Because human estrogenic 17 beta-hydroxysteroid dehydrogenase (HSD17B1) catalyzes the last step in the biosynthesis of 17 beta-estradiol from the less potent estrogen, estrone, it is an attractive target for the design of inhibitors of estrogen production and tumor growth (1). It is concluded that the steroid-binding site of human placental HSD17B1 contains a histidine residue which proximates the upper A-ring region of the steroid as it undergoes the reversible binding step (2). Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies (3). Defects in the conversion of androstenedione to testosterone in the fetal testes by the enzyme HSD17B1 give rise to genetic males with female external genitalia (4).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:40\nWestern Blot: 1:500\nImmunohistochemistry: 1:100-1:250\nImmunocytochemistry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

H9117-83A

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant.

Isotype

IgG

References

1. Murdock GL, et al. J Biol Chem 258(19):11460-4, 1983. 2. Ghosh D, et al. Structure 3(5):503-13, 1995. 3. Huang YW, et al. Mol Endocrinol 15(11):2010-20, 2001 4. Geissler WM, et al. Nat Genet 7(1):34-9, 1994.

Additional Info

Recognizes human HSD17B1 at ~35kD.