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Hygromycin B 50mg/ml Solution

Hygromycin B 50mg/ml Solution

Cat no: H9700

Supplier: United States Biological
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Hygromycin B is an aminoglycosidic antibiotic which inhibits protein synthesis in bacteria, fungi and mammalian cells and is used to select prokaryotic and eukaryotic cells that have been transfected with the Hygromycin gene (hyg, hph, HMA, HM+). Source: Streptomyces hygroscopicus Purity (HPLC): (same/more than) 76% Bioassay: 300-500units/mg solution Density: 1.13g/ml Concentration: 50mg Hygromycin B/ml Form: Supplied as a liquid. Prepared with RNase, DNase-free, molecular biology grade water (W0900). Sterile-filtered (0.2um). Recommended Concentration Range: 25-1500ug/ml E.coli JM 83: 25-200ug/ml Human: 50-400ug/ml Saccharomyces cerevisiae: 200ug/ml Chicken: 1500ug/ml Tobacco: 20-200ug/ml Storage and Stability: May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: H9700
Size: 10ml
Alternative names: Streptomyces hygroscopicus
References: US Biological application reference: Kojic, M. and Holloman, W.K. (2000) Can J Microbiol. 46:333-338. 1. Hamada, W., et al. 1994. Curr. Genetics 26, 251. 2. Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731. 3. Gaken, J., et al. 1992. Biotechniques 13, 32. 4. Ma, H., et al. 1992. Gene 117, 161. 5. Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932. 6. Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53. 7. McIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67. 8. Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.

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