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Hypoxia Inducible Factor 1 alpha, Recombinant, Human (HIF-1a)

Cat no: H9804-01B

Hypoxia Inducible Factor 1 alpha, Recombinant, Human (HIF-1a)

Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1beta subunit and one of three subunits (HIF-1alpha, HIF-2alpha or HIF-3alpha). The activation of HIF-1alpha is closely associated with a variety of tumors and oncogenic pathways. HIF-1alpha consists of DNA binding domain (DBD domain), Dimerization domain and C-terminal regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. HIF-1alpha (aa530-826) contains two TAD and inhibitory domain.\n\nAmino Acid Sequence:\nMEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP\nKILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ\nKTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H9804-01B

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 1mM DTT

Purity

(same/more than) 95% by SDS-PAGE. Purified by conventional chromatography techniques.

References

1. Okuyama, H., et al., J. Biol. Chem. 281(22): 15,554-15,563 (2006). 2. Berra, E., et al., EMBO 7(1): 41-45 (2006).

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