Hypoxia Inducible Factor 1 beta (HIF-1b, ARNT, Aryl hydrocarbon Receptor Nuclear Translocator)

ARNT (Aryl hydrocarbon nuclear translocator; also HIF-1 beta) is an 87kD member of the \nbHLH-PAS subclass of the bHLH family of transcription factors. It is widely expressed, and \nserves as part of both the NF kappa B and aryl hydrocarbon receptor (AHR) signaling nodes. For the AHR, the AHR is normally inactive and bound to hsp90 in the cytosol. Following ligand binding, the AHR complex enters the nucleus, dissociates from hsp90, dimerizes with ARNT, and then binds response elements of select genes. Human ARNT is 789aa in length. It contains a DNA-binding region (aa #87-143), two PAS domains (aa161-235 and 362-458) and one PAC region (aa424-467). There are multiple potential splice forms. Two alternate start sites are found at Met10 and Met354, there is a deletion of aa329-755 and 77-91, and an Ala substitution for aa 328-789. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1.0ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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