Hypoxia Inducible Factor 1a (HIF-1a)

Hypoxia-inducible factor-1 (HIF-1). HIF-1 is a heterodimer consisting of an alpha and a beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF-1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron chelation. HIF-1 activates transcription of hypoxia-inducible genes, including those encoding: erythropoietin, vascular endothelial growth (VEGF), heme oxygenase-1, inducible nitric oxide synthase, and the glycolytic enzymes aldolase A, enolase 1, lactate dehydrogenase A, phosphofructokinase I, and phosphoglycerate kinase 1. The C-terminal of HIF-1 alpha binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF.\n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: paraffin\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMammalian cells cultured under reduced O2 tension.\n\nCellular Localization: \nNuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H9807

Size

500ul

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Sheep

Form

Supplied as a liquid in PBS, pH 7.4, 0.2%BSA, 0.1% sodium azide, before the addition of 40% glycerol. Also available without BSA and azide. See cat# H9807X.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b,k

References

1. Semenza, GL, Hypoxia-inducible factor 1 and the molecular physiology of oxygen homeostasis. J. Lab Clinical Medicine. 131: 207-214, 1998.\n2. Iyer, NV, et al. Cellular and developmental control of 02 homeostasis by hypoxia-inducible factor alpha. Genes and Development. 12(2): 149-162, 1998.\n3. Flamme I; Frohlich T; von Reutern M; Kappel A; Damert A; Risau W. HRF, a putative basic helix-loop-helix-PAS-domain transcription factor is closely related to hypoxia-inducible factor-1 alpha and developmentally expressed in blood vessels. Mechanisms of Development, 1997, 63(1):51-60.\n4. Gassmann M; Kvietikova I; Rolfs A; Wenger RH. Oxygen-and dioxin-regulated gene expression in mouse hepatoma cells. Kidney International, 1997 Feb, 51(2):567-74.\n5. Huang LE; Ho V; Arany Z; Krainc D; Galson D; Tendler D; Livingston DM; Bunn HF. Erythropoietin gene regulation depends on heme-dependent oxygen sensing and assembly of interacting transcription factors. Kidney International, 1997 Feb, 51(2):548-52.\n6. Jelkmann W; Pagel H; Hellwig T; Fandrey J. Effects of antioxidant vitamins on renal and hepatic erythropoietin production. Kidney International, 1997 Feb, 51(2):497-501.\n7. Pugh CW; O

Additional Info

Recognizes the alpha subunit of HIF-1 at 120kD. Species crossreactivity: Human, sheep, mouse, rat and ferret.