Hypoxia Inducible Factor 1a (HIF-1a)

Hypoxia Inducible Factor 1 (HIF-1) is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot:1:500-1:1000\nImmunoprecipitation: 1:1000\nChromatin Immunoprecipitation: 25ul for 15 million cells. Nuclear extracts are recommended.\nImmunohistochemistry (Paraffin): 1:100. Mixed results with mouse.\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nRCC4 lysates, COS-7 hypoxic induced control lysate, PC12 nuclear extracts\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H9807-04

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Raina S, et al. Anti-VEGF treatment accelerates polycystic kidney disease. Am J Physiol Renal Physiol. 2011 Jun 15.[PMID 21677148]. 2. Wang Y, et al. MicroRNA-138 modulates DNA damage response by repressing histone H2AX expression. Mol Cancer Res. 2011 Jun 21.[PMID 21693595]. 3. Belibi F, et al. Hypoxia-inducible factor 1{alpha} (HIF-1 {alpha}) and autophagy in polycystic kidney disease (PKD). Am J Physiol Renal Physiol. 2011.[PMID 21270095]. 4. Filby CE, et al. Respir Res. 2010 Apr 23;11(1):42. 5. Xia X, Kung AL. Preferential binding of HIF-1 to transcriptionally active loci determines cell-type specific response to hypoxia (BioMed Central). Genome Biol (2009). [PMID: 19828020]. 6. Frede S, et al. J Immunol. 2009 May 15;182(10):6470-6. [PMID: 19414801]. 7. Rane S, et al. Circ Res. 2009 Apr 10;104(7):879-86. 8. Naito Y, et al. Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H585-93. 9. Botusan IR, et al. Proc Natl Acad Sci USA. 2008 Dec 9;105(49):19426-31. 10. Land SC, Wilson SM. Antioxid Redox Signal. 2005 Jan-Feb;7(1-2):92-107. 11. Westfall SD, et al. Stem Cells Dev. 2008 Oct;17(5):869-81. 12. Trollmann R, et al. Am J Physiol Regul Integr Comp Physiol. 2008 Dec;295(6):R1973-81. 13. Meyuhas R, et al. Mol Cancer Res. 2008 Sep;6(9):1397-409. 14. Nikinmaa M, et al. J Exp Biol. 2008 Jun;211(Pt 12):1999-2004. 15. Zhang H, et al. J Biol Chem. 2008 Apr 18;283(16):10892-903. 16. Piantadosi CA, Suliman HB. J Biol Chem. 2008 Apr 18;283(16):10967-77. 17. Ohtomo S, et al. Nephrol Dial Transplant. 2008 Apr;23(4):1166-72. 18. Lauzier MC, et al. Mol Pharmacol. 2008 Jul;74(1):282-8. 19. Singh RP, Gu M, Agarwal R. Cancer Res. 2008 Mar 15;68(6):2043-50. 20. Sikder D, Kodadek T. Genes Dev. 2007 Nov 15;21(22):2995-3005.

Additional Info

Recognizes human Hypoxia Inducible Factor 1a. Species Crossreactivity: Mouse, rat, primate