Hypoxia Inducible Factor 1a (HIF-1a)

Hypoxia, a condition of low tissue O2 concentration, plays an important role in normal physiological processes and tumor formation. Under hypoxic conditions mammalian cells up regulate the expression of hypoxic genes, including induction of angiogenesis and a switch to anaerobic metabolism, in order to survive. HIF-1 (Hypoxia Inducible Factor-1) is one of the key regulators of the transcriptional response to oxygen deprivation (1). HIF-1 is composed of two subunits, HIF-1a and HIF-1b also known as aryl hydrocarbon receptor nuclear translocator (ARNT)) that are members of the basic helix-loop-helix (bHLH) Per-Arnt-Sim (PAS) (bHLH-PAS) family of transcription factors. HIF-1 is essential for angiogenesis, embryonic development, and is associated with tumor progression, erythropoiesis, vascular development/remodeling, vasodilation, and glucose/energy metabolism. The over expression of HIF-1a has been demonstrated in many common human cancers including prostate and breast, in which HIF-1a levels are associated with increase vascularitry and tumor progression. Besides physiological hypoxia, genetic abnormalities frequently detected in human cancers, such as loss of function mutations (Von Hippel-Lindau, p53, and PTEN), are associated with induction of HIF1 activity and expression of HIF-1-inducible genes (1).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000 \nImmunohistochemistry (paraffin): 1:100-250\nImmunocytochemistry: 1:100-250\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRamos + CoCl2 lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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