Hypoxia Inducible Factor 1b (Hypoxia-inducible Factor 1-beta, HIF1beta, HIF1-beta, HIF-1beta, HIF-1-beta, HIF1B, Aryl Hydrocarbon Receptor Nuclear Translocator, ARNT Protein, Class E Basic Helix-loop-helix Protein 2, bHLHe2, Dioxin Receptor Nuclear Transl

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role\nin the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1a and HIF-1b, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1a is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1a is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3,4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1a degradation and lead to its stabilization. In addition, HIF-1a can be induced\nin an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7). HIF-1b is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1b plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1b expression levels decrease\nsignificantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1b plays an important role in pancreatic b-cell function (10).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunohistochemistry: 1:50\nImmunoprecipitation (Paraffin): 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H9808-04A

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Sharp, F.R. and Bernaudin, M. (2004) Nat Rev Neurosci 5, 437-48. (2) Wang, G.L. et al. (1995) Proc Natl Acad Sci USA 92, 5510-4. (3) Jaakkola, P. et al. (2001) Science 292, 468-72. (4) Maxwell, P.H. et al. (1999) Nature 399, 271-5. (5) Fukuda, R. et al. (2002) J Biol Chem 277, 38205-11. (6) Jiang, B.H. et al. (2001) Cell Growth Differ 12, 363-9. (7) Laughner, E. et al. (2001) Mol Cell Biol 21, 3995-4004. (8) Walisser, J.A. et al. (2004) Proc Natl Acad Sci USA 101, 16677-82. (9) Salomon-Nguyen, F. et al. (2000) Proc Natl Acad Sci USA 97, 6757-62. (10) Gunton, J.E. et al. (2005) Cell 122, 337-49.

Additional Info

Recognizes endogenous levels of total HIF-1b/ARNT. Species Crossreactivity: monkey, mouse, rat.