Hypoxia Up-regulated Protein 1 (HYOU1, 150kD Oxygen-regulated Protein, ORP150, ORP-150, 170kD Glucose-regulated Protein, Grp170, GRP-170, DKFZp686N08236, FLJ94899, FLJ97572, HSP12A) (Azide Free)

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.\n\nApplications:\nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1.5ug/ml\nELISA: 1ug/ml-3ng/ml\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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